Journal: Regenerative Therapy

Article Title: WTAP stabilizes MMP12 expression to promote the malignant phenotypes of esophageal cancer cells

doi: 10.1016/j.reth.2026.101101

Figure Lengend Snippet: MMP12 silencing inhibited M2 macrophage polarization. THP-1 cells were differentiated into M0 macrophages (THP-1 M0) by treatment with 100 ng/mL PMA for 24 h. (A) Flow cytometry was used to quantify the number of CD68-positive cells. Subsequently, KYSE150 cells were co-cultured with the THP-1-derived macrophages using a Transwell system. (B) The mRNA levels of IL-10, Arg-1, and TGF-β were detected by qRT-PCR. (C) Flow cytometry was used to quantify the number of CD206-positive macrophages. (D) Cell migration analysis by transwell migration assay. ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001.

Article Snippet: For immunophenotyping, single-cell suspensions of both THP-1 and THP-1-M0 cells were labeled with an anti-CD68 antibody (E-AB-F1299L, Elabscience, Wuhan, China).

Techniques: Flow Cytometry, Cell Culture, Derivative Assay, Quantitative RT-PCR, Migration, Transwell Migration Assay

Journal: International Dental Journal

Article Title: Immunological Features of Peri-Implant Soft Tissue After Healing Abutment Dislodgement: A Comparative Human Study

doi: 10.1016/j.identj.2026.109521

Figure Lengend Snippet: Immunohistochemical staining for immune cell markers in the epithelial + superficial connective tissue region. Representative immunohistochemical images of the superficial peri-implant soft tissue zone stained for MPO, CD68, CD3, CD20, and CD138. Scale bars: 100 μm. Red dashed boxes and red arrows indicate the magnified regions (insets).

Article Snippet: Sections were then incubated overnight at 4 °C with primary antibodies (MPO, CD68, CD3, CD20 and CD138, all purchased from Boster, China; dilution 1:400).

Techniques: Immunohistochemical staining, Staining